The main objective is to utilize sugar beet pulp residues from sugar industry to produce cellulose nanofibers (CNF) after extraction pectin. The isolated CNF was comprehensively characterized regarding its purity, chemical composition, in addition to spectroscopic analyses tools such as X-ray diffraction (XRD), Fourier transform infrared (FTIR), atomic force microscopy (AFM) for testing their surface roughness, transmission electron microscopy (TEM) to prove the dimensions of the isolated nanofibers, as well as mechanical properties of CNF thin films. The isolated CNF has many applications; for example it was successfully tested as a coating material for paper products to improve mechanical and barrier properties of paper and impart it good anti-grease properties. Applications in different food products as a thickener and as fat replacement in low-fact product. In non-food products such coating materials in paper industry.